2LSN

Solution structure of PFV RNase H domain

2LSN の概要

• エントリーDOI: 10.2210/pdb2lsn/pdb
• NMR情報: BMRB: 17745
• 分子名称: RNase H (1 entity in total)
• 機能のキーワード: rnase h, viral protein
• 由来する生物種: Human spumaretrovirus (SFVcpz(hu), Prototype foamy virus)
• 細胞内の位置: Integrase: Virion (Potential). Protease/Reverse transcriptase/ribonuclease H: Host nucleus (By similarity): P14350
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18056.52

構造登録者

Leo, B.,Schweimer, K.,Woehrl, B. (登録日: 2012-05-03, 公開日: 2012-10-17, 最終更新日: 2024-05-15)

主引用文献

Leo, B.,Schweimer, K.,Rosch, P.,Hartl, M.J.,Wohrl, B.M.
The solution structure of the prototype foamy virus RNase H domain indicates an important role of the basic loop in substrate binding.
Retrovirology, 9:73-73, 2012

PubMed Abstract: The ribonuclease H (RNase H) domains of retroviral reverse transcriptases play an essential role in the replication cycle of retroviruses. During reverse transcription of the viral genomic RNA, an RNA/DNA hybrid is created whose RNA strand needs to be hydrolyzed by the RNase H to enable synthesis of the second DNA strand by the DNA polymerase function of the reverse transcriptase. Here, we report the solution structure of the separately purified RNase H domain from prototype foamy virus (PFV) revealing the so-called C-helix and the adjacent basic loop, which both were suggested to be important in substrate binding and activity.

PubMed: 22962864
DOI: 10.1186/1742-4690-9-73

実験手法

SOLUTION NMR