2LSL

Solution structure of the C-terminal domain of Tetrahymena telomerase protein p65

2LSL の概要

• エントリーDOI: 10.2210/pdb2lsl/pdb
• 関連するPDBエントリー: 4ERD 4EYT
• NMR情報: BMRB: 18435
• 分子名称: Telomerase associated protein p65 (1 entity in total)
• 機能のキーワード: telomerase, p65, la protein, larp7, rrm, tetrahymena, rna binding protein
• 由来する生物種: Tetrahymena thermophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16191.28

構造登録者

Singh, M.,Wang, Z.,Koo, B.,Patel, A.,Cascio, D.,Collins, K.,Feigon, J. (登録日: 2012-05-01, 公開日: 2012-06-20, 最終更新日: 2024-05-15)

主引用文献

Singh, M.,Wang, Z.,Koo, B.K.,Patel, A.,Cascio, D.,Collins, K.,Feigon, J.
Structural Basis for Telomerase RNA Recognition and RNP Assembly by the Holoenzyme La Family Protein p65.
Mol.Cell, 47:16-26, 2012

PubMed Abstract: Telomerase is a ribonucleoprotein complex essential for maintenance of telomere DNA at linear chromosome ends. The catalytic core of Tetrahymena telomerase comprises a ternary complex of telomerase RNA (TER), telomerase reverse transcriptase (TERT), and the essential La family protein p65. NMR and crystal structures of p65 C-terminal domain and its complex with stem IV of TER reveal that RNA recognition is achieved by a combination of single- and double-stranded RNA binding, which induces a 105° bend in TER. The domain is a cryptic, atypical RNA recognition motif with a disordered C-terminal extension that forms an α helix in the complex necessary for hierarchical assembly of TERT with p65-TER. This work provides the first structural insight into biogenesis and assembly of TER with a telomerase-specific protein. Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets.

PubMed: 22705372
DOI: 10.1016/j.molcel.2012.05.018

実験手法

SOLUTION NMR