2LS0

Solution Structure of the Target Recognition Domain of Zoocin A

2LS0 の概要

• エントリーDOI: 10.2210/pdb2ls0/pdb
• NMR情報: BMRB: 18404
• 分子名称: Zoocin A endopeptidase (1 entity in total)
• 機能のキーワード: specificity, hydrolase
• 由来する生物種: Streptococcus equi subsp. zooepidemicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14089.65

構造登録者

Timkovich, R.,Chen, Y.,Simmonds, R.S. (登録日: 2012-04-17, 公開日: 2012-12-05, 最終更新日: 2024-05-01)

主引用文献

Chen, Y.,Simmonds, R.S.,Young, J.K.,Timkovich, R.
Solution structure of the recombinant target recognition domain of zoocin A.
Proteins, 81:722-727, 2013

PubMed Abstract: The protein rTRD is the recombinant form of the target recognition domain of zoocin A, a lytic exoenzyme produced by Streptococcus equi subspecies zooepidemicus 4881. It has no known sequence homologs. However, the catalytic domain of zoocin A is homologous to lysostaphin which is another exoenzyme active against a different spectrum of bacteria, including the pathogen Staphylococcus aureus. An ensemble of models for the solution structure of rTRD has been generated by NMR techniques. The minimum energy model from the ensemble was subjected to three-dimensional homology search engines, but no homologs were found, suggesting rTRD may represent a new protein folding family. There is some similarity in the folding of rTRD to the immunoglobin fold of the antigen binding region of mammalian antibodies which could suggest an ancient evolutionary relation.

PubMed: 23184858
DOI: 10.1002/prot.24224

実験手法

SOLUTION NMR