2LRS

The second dsRBD domain from A. thaliana DICER-LIKE 1

2LRS の概要

• エントリーDOI: 10.2210/pdb2lrs/pdb
• NMR情報: BMRB: 18393
• 分子名称: Endoribonuclease Dicer homolog 1 (1 entity in total)
• 機能のキーワード: mirna, rna binding, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• 細胞内の位置: Nucleus: Q9SP32
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7961.23

構造登録者

Burdisso, P.,Suarez, I.,Bersch, B.,Bologna, N.,Palatnik, J.,Boisbouvier, J.,Rasia, R. (登録日: 2012-04-12, 公開日: 2013-01-23, 最終更新日: 2024-05-01)

主引用文献

Burdisso, P.,Suarez, I.P.,Bologna, N.G.,Palatnik, J.F.,Bersch, B.,Rasia, R.M.
Second Double-Stranded RNA Binding Domain of Dicer-like Ribonuclease 1: Structural and Biochemical Characterization.
Biochemistry, 51:10159-10166, 2012

PubMed Abstract: Dicer-like ribonuclease III enzymes are involved in different paths related to RNA silencing in plants. Little is known about the structural aspects of these processes. Here we present a structural characterization of the second double-stranded RNA binding domain (dsRBD) of DCL1, which is presumed to participate in pri-micro-RNA recognition and subcellular localization of this protein. We determined the solution structure and found that it has a canonical fold but bears some variation with respect to other homologous domains. We also found that this domain binds both double-stranded RNA and double-stranded DNA, in contrast to most dsRBDs. Our characterization shows that this domain likely has functions other than substrate recognition and binding.

PubMed: 23194006
DOI: 10.1021/bi301247r

実験手法

SOLUTION NMR