2LRE

The solution structure of the dimeric Acanthaporin

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2LRE の概要

• エントリーDOI: 10.2210/pdb2lre/pdb
• 関連するPDBエントリー: 2LRD
• NMR情報: BMRB: 18358
• 分子名称: Acanthaporin (1 entity in total)
• 機能のキーワード: pore-forming toxin, alpha helix, antimicrobial protein
• 由来する生物種: Acanthamoeba culbertsoni
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12002.46

構造登録者

Michalek, M.,Soennichsen, F.D.,Wechselberger, R.,Dingley, A.J.,Wienk, H.,Simanski, M.,Herbst, R.,Lorenzen, I.,Marciano-Cabral, F.,Gelhaus, C.,Groetzinger, J.,Leippe, M. (登録日: 2012-03-28, 公開日: 2012-05-02, 最終更新日: 2023-06-14)

主引用文献

Michalek, M.,Sonnichsen, F.D.,Wechselberger, R.,Dingley, A.J.,Hung, C.W.,Kopp, A.,Wienk, H.,Simanski, M.,Herbst, R.,Lorenzen, I.,Marciano-Cabral, F.,Gelhaus, C.,Gutsmann, T.,Tholey, A.,Grotzinger, J.,Leippe, M.
Structure and function of a unique pore-forming protein from a pathogenic acanthamoeba.
Nat.Chem.Biol., 9:37-42, 2013

PubMed Abstract: Human pathogens often produce soluble protein toxins that generate pores inside membranes, resulting in the death of target cells and tissue damage. In pathogenic amoebae, this has been exemplified with amoebapores of the enteric protozoan parasite Entamoeba histolytica. Here we characterize acanthaporin, to our knowledge the first pore-forming toxin to be described from acanthamoebae, which are free-living, bacteria-feeding, unicellular organisms that are opportunistic pathogens of increasing importance and cause severe and often fatal diseases. We isolated acanthaporin from extracts of virulent Acanthamoeba culbertsoni by tracking its pore-forming activity, molecularly cloned the gene of its precursor and recombinantly expressed the mature protein in bacteria. Acanthaporin was cytotoxic for human neuronal cells and exerted antimicrobial activity against a variety of bacterial strains by permeabilizing their membranes. The tertiary structures of acanthaporin's active monomeric form and inactive dimeric form, both solved by NMR spectroscopy, revealed a currently unknown protein fold and a pH-dependent trigger mechanism of activation.

PubMed: 23143413
DOI: 10.1038/nchembio.1116

実験手法

SOLUTION NMR