2LR5

1H chemical shift assignments for micasin

2LR5 の概要

• エントリーDOI: 10.2210/pdb2lr5/pdb
• NMR情報: BMRB: 18347
• 分子名称: micasin (1 entity in total)
• 機能のキーワード: antimicrobial protein
• 由来する生物種: Arthroderma otae (Microsporum canis)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4067.70

構造登録者

Harvey, P.J.,Craik, D.J.,Zhu, S. (登録日: 2012-03-22, 公開日: 2012-06-27, 最終更新日: 2024-11-13)

主引用文献

Zhu, S.,Gao, B.,Harvey, P.J.,Craik, D.J.
Dermatophytic defensin with antiinfective potential
Proc.Natl.Acad.Sci.USA, 109:8495-8500, 2012

PubMed Abstract: Fungi are a newly emerging source of peptide antibiotics with therapeutic potential. Here, we report 17 new fungal defensin-like peptide (fDLP) genes and the detailed characterization of a corresponding synthetic fDLP (micasin) from a dermatophyte in terms of its structure, activity and therapeutic potential. NMR analysis showed that synthetic micasin adopts a "hallmark" cysteine-stabilized α-helical and β-sheet fold. It was active on both gram-positive and gram-negative bacteria, and importantly it killed two clinical isolates of methicillin-resistant Staphylococcus aureus and the opportunistic pathogen Pseudomonas aeruginosa at low micromolar concentrations. Micasin killed approximately 100% of treated bacteria within 3 h through a membrane nondisruptive mechanism of action, and showed extremely low hemolysis and high serum stability. Consistent with these functional properties, micasin increases survival in mice infected by the pathogenic bacteria in a peritonitis model. Our work represents a valuable approach to explore novel peptide antibiotics from a large resource of fungal genomes.

PubMed: 22586077
DOI: 10.1073/pnas.1201263109

実験手法

SOLUTION NMR