2LQZ

Structure of the RNA claw of the DNA packaging motor of bacteriophage 29

2LQZ の概要

• エントリーDOI: 10.2210/pdb2lqz/pdb
• NMR情報: BMRB: 18336
• 分子名称: RNA (27-MER) (1 entity in total)
• 機能のキーワード: rna, bulge
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8586.13

構造登録者

Harjes, E.J.,Matsuo, H.J.,Kitamura, A.J. (登録日: 2012-03-19, 公開日: 2012-08-01, 最終更新日: 2024-05-15)

主引用文献

Harjes, E.,Kitamura, A.,Zhao, W.,Morais, M.C.,Jardine, P.J.,Grimes, S.,Matsuo, H.
Structure of the RNA claw of the DNA packaging motor of bacteriophage 29.
Nucleic Acids Res., 40:9953-9963, 2012

PubMed Abstract: Bacteriophage DNA packaging motors translocate their genomic DNA into viral heads, compacting it to near-crystalline density. The Bacillus subtilis phage 29 has a unique ring of RNA (pRNA) that is an essential component of its motor, serving as a scaffold for the packaging ATPase. Previously, deletion of a three-base bulge (18-CCA-20) in the pRNA A-helix was shown to abolish packaging activity. Here, we solved the structure of this crucial bulge by nuclear magnetic resonance (NMR) using a 27mer RNA fragment containing the bulge (27b). The bulge actually involves five nucleotides (17-UCCA-20 and A100), as U17 and A100 are not base paired as predicted. Mutational analysis showed these newly identified bulge residues are important for DNA packaging. The bulge introduces a 33-35° bend in the helical axis, and inter-helical motion around this bend appears to be restricted. A model of the functional 120b pRNA was generated using a 27b NMR structure and the crystal structure of the 66b prohead-binding domain. Fitting this model into a cryo-EM map generated a pentameric pRNA structure; five helices projecting from the pRNA ring resemble an RNA claw. Biochemical analysis suggested that this shape is important for coordinated motor action required for DNA translocation.

PubMed: 22879380
DOI: 10.1093/nar/gks724

実験手法

SOLUTION NMR