2LPZ

Atomic model of the Type-III Secretion System Needle

2LPZ の概要

• エントリーDOI: 10.2210/pdb2lpz/pdb
• 分子名称: Protein prgI (1 entity in total)
• 機能のキーワード: type three secretion system, filament, needle, helical assembly, transport protein
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 29
• 化学式量合計: 257081.17

構造登録者

Loquet, A.,Sgourakis, N.G.,Gupta, R.,Giller, K.,Riedel, D.,Goosmann, C.,Griesinger, C.,Kolbe, M.G.,Baker, D.,Becker, S.,Lange, A. (登録日: 2012-02-21, 公開日: 2012-05-16, 最終更新日: 2024-05-15)

主引用文献

Loquet, A.,Sgourakis, N.G.,Gupta, R.,Giller, K.,Riedel, D.,Goosmann, C.,Griesinger, C.,Kolbe, M.,Baker, D.,Becker, S.,Lange, A.
Atomic model of the type III secretion system needle.
Nature, 486:276-279, 2012

PubMed Abstract: Pathogenic bacteria using a type III secretion system (T3SS) to manipulate host cells cause many different infections including Shigella dysentery, typhoid fever, enterohaemorrhagic colitis and bubonic plague. An essential part of the T3SS is a hollow needle-like protein filament through which effector proteins are injected into eukaryotic host cells. Currently, the three-dimensional structure of the needle is unknown because it is not amenable to X-ray crystallography and solution NMR, as a result of its inherent non-crystallinity and insolubility. Cryo-electron microscopy combined with crystal or solution NMR subunit structures has recently provided a powerful hybrid approach for studying supramolecular assemblies, resulting in low-resolution and medium-resolution models. However, such approaches cannot deliver atomic details, especially of the crucial subunit-subunit interfaces, because of the limited cryo-electron microscopic resolution obtained in these studies. Here we report an alternative approach combining recombinant wild-type needle production, solid-state NMR, electron microscopy and Rosetta modelling to reveal the supramolecular interfaces and ultimately the complete atomic structure of the Salmonella typhimurium T3SS needle. We show that the 80-residue subunits form a right-handed helical assembly with roughly 11 subunits per two turns, similar to that of the flagellar filament of S. typhimurium. In contrast to established models of the needle in which the amino terminus of the protein subunit was assumed to be α-helical and positioned inside the needle, our model reveals an extended amino-terminal domain that is positioned on the surface of the needle, while the highly conserved carboxy terminus points towards the lumen.

PubMed: 22699623
DOI: 10.1038/nature11079

実験手法

SOLID-STATE NMR