2LP4
Solution structure of P1-CheY/P2 complex in bacterial chemotaxis
Summary for 2LP4
| Entry DOI | 10.2210/pdb2lp4/pdb |
| Related | 1EAY 1I5N |
| NMR Information | BMRB: 18234 |
| Descriptor | Chemotaxis protein CheA, Chemotaxis protein CheY (2 entities in total) |
| Functional Keywords | two component signaling system, histidine phosphotransfer domain, response regulator, transferase-signaling protein complex, transferase/signaling protein |
| Biological source | Escherichia coli More |
| Cellular location | Cytoplasm: P07363 P0AE67 |
| Total number of polymer chains | 2 |
| Total formula weight | 38971.98 |
| Authors | Dahlquist, F.,Mo, G.,Zhou, H.,Kamamura, T. (deposition date: 2012-01-31, release date: 2012-10-24, Last modification date: 2024-05-15) |
| Primary citation | Mo, G.,Zhou, H.,Kawamura, T.,Dahlquist, F.W. Solution structure of a complex of the histidine autokinase CheA with its substrate CheY. Biochemistry, 51:3786-3798, 2012 Cited by PubMed Abstract: In the bacterial chemotaxis two-component signaling system, the histidine-containing phosphotransfer domain (the "P1" domain) of CheA receives a phosphoryl group from the catalytic domain (P4) of CheA and transfers it to the cognate response regulator (RR) CheY, which is docked by the P2 domain of CheA. Phosphorylated CheY then diffuses into the cytoplasm and interacts with the FliM moiety of the flagellar motors, thereby modulating the direction of flagellar rotation. Structures of various histidine phosphotransfer domains (HPt) complexed with their cognate RR domains have been reported. Unlike the Escherichia coli chemotaxis system, however, these systems lack the additional domains dedicated to binding to the response regulators, and the interaction of an HPt domain with an RR domain in the presence of such a domain has not been examined on a structural basis. In this study, we used modern nuclear magnetic resonance techniques to construct a model for the interaction of the E. coli CheA P1 domain (HPt) and CheY (RR) in the presence of the CheY-binding domain, P2. Our results indicate that the presence of P2 may lead to a slightly different relative orientation of the HPt and RR domains versus those seen in such complex structures previously reported. PubMed: 22494339DOI: 10.1021/bi300147m PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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