2LP0

The solution structure of homeodomain-protein complex

2LP0 の概要

• エントリーDOI: 10.2210/pdb2lp0/pdb
• NMR情報: BMRB: 17407
• 分子名称: Homeobox protein Hox-C9, Geminin (2 entities in total)
• 機能のキーワード: homeodomain, transcription-cell cycle complex, transcription/cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P31274; Cytoplasm: O75496
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10069.45

構造登録者

Liu, C.,Zhou, B.,Xu, Z.,Zhu, G. (登録日: 2012-01-29, 公開日: 2012-06-06, 最終更新日: 2024-05-15)

主引用文献

Zhou, B.,Liu, C.,Xu, Z.,Zhu, G.
Structural basis for homeodomain recognition by the cell-cycle regulator Geminin
Proc.Natl.Acad.Sci.USA, 2012

PubMed Abstract: Homeodomain-containing transcription factors play a fundamental role in the regulation of numerous developmental and cellular processes. Their multiple regulatory functions are accomplished through context-dependent inputs of target DNA sequences and collaborating protein partners. Previous studies have well established the sequence-specific DNA binding to homeodomains; however, little is known about how protein partners regulate their functions through targeting homeodomains. Here we report the solution structure of the Hox homeodomain in complex with the cell-cycle regulator, Geminin, which inhibits Hox transcriptional activity and enrolls Hox in cell proliferative control. Side-chain carboxylates of glutamates and aspartates in the C terminus of Geminin generate an overall charge pattern resembling the DNA phosphate backbone. These residues provide electrostatic interactions with homeodomain, which combine with the van der Waals contacts to form the stereospecific complex. We further showed that the interaction with Geminin is homeodomain subclass-selective and Hox paralog-specific, which relies on the stapling role of residues R43 and M54 in helix III and the basic amino acid cluster in the N terminus. Interestingly, we found that the C-terminal residue Ser184 of Geminin could be phosphorylated by Casein kinase II, resulting in the enhanced binding to Hox and more potent inhibitory effect on Hox transcriptional activity, indicating an additional layer of regulation. This structure provides insight into the molecular mechanism underlying homeodomain-protein recognition and may serve as a paradigm for interactions between homeodomains and DNA-competitive peptide inhibitors.

PubMed: 22615398
DOI: 10.1073/pnas.1200874109

実験手法

SOLUTION NMR