2LON

Backbone structure of human membrane protein HIGD1B

2LON の概要

• エントリーDOI: 10.2210/pdb2lon/pdb
• 関連するPDBエントリー: 2LOM 2LOO 2LOP 2LOQ
• NMR情報: BMRB: 18218
• 分子名称: HIG1 domain family member 1B (1 entity in total)
• 機能のキーワード: membrane protein, helical bundle
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Multi-pass membrane protein (Potential): Q9P298
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11074.96

構造登録者

Klammt, C.,Maslennikov, I.,Kwiatkowski, W.,Choe, S. (登録日: 2012-01-26, 公開日: 2012-05-23, 最終更新日: 2024-05-15)

主引用文献

Klammt, C.,Maslennikov, I.,Bayrhuber, M.,Eichmann, C.,Vajpai, N.,Chiu, E.J.,Blain, K.Y.,Esquivies, L.,Kwon, J.H.,Balana, B.,Pieper, U.,Sali, A.,Slesinger, P.A.,Kwiatkowski, W.,Riek, R.,Choe, S.
Facile backbone structure determination of human membrane proteins by NMR spectroscopy.
Nat.Methods, 9:834-839, 2012

PubMed Abstract: Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.

PubMed: 22609626
DOI: 10.1038/nmeth.2033

実験手法

SOLUTION NMR