2LO0

Solution structure of the Get5 carboxyl domain from A. fumigatus

2LO0 の概要

• エントリーDOI: 10.2210/pdb2lo0/pdb
• 関連するPDBエントリー: 2lnz 3VEJ
• NMR情報: BMRB: 18187
• 分子名称: Uncharacterized protein (1 entity in total)
• 機能のキーワード: dimerization, homodimerization, protein binding
• 由来する生物種: Aspergillus fumigatus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16516.06

構造登録者

Chartron, J.W.,Vandervelde, D.G.,Rao, M.,Clemons Jr., W.M. (登録日: 2012-01-08, 公開日: 2012-01-25, 最終更新日: 2024-05-15)

主引用文献

Chartron, J.W.,Vandervelde, D.G.,Rao, M.,Clemons, W.M.
Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.
J.Biol.Chem., 287:8310-8317, 2012

PubMed Abstract: Tail-anchored trans-membrane proteins are targeted to membranes post-translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of tail-anchored proteins destined to the endoplasmic reticulum from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog, and comparison of these structures allows annotation of specific stabilizing interactions. Using solution x-ray scattering and the structures of all folded domains, we present a model of the full-length Get4/Get5 complex.

PubMed: 22262836
DOI: 10.1074/jbc.M111.333252

実験手法

SOLUTION NMR