2LNE

Neurotensin 40 structures in water pH 5.5 298 K. NMR data & structures

2LNE の概要

• エントリーDOI: 10.2210/pdb2lne/pdb
• 関連するPDBエントリー: 2LNF 2LNG
• NMR情報: BMRB: 18162
• 分子名称: Neurotensin (1 entity in total)
• 機能のキーワード: neurotensin(nt), recombinant technology, neuropeptide
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P30990
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1693.96

構造登録者

Mukhopadhyay, C.,Khatun, U. (登録日: 2011-12-27, 公開日: 2012-11-14, 最終更新日: 2024-05-15)

主引用文献

Khatun, U.L.,Goswami, S.K.,Mukhopadhyay, C.
Modulation of the neurotensin solution structure in the presence of ganglioside GM1 bicelle.
Biophys.Chem., 168:48-59, 2012

PubMed Abstract: Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that shows multiple biological function in central and peripheral nervous systems. Gangliosides are glycosphingolipids, most abundant in the plasma membrane of nerve cells. Here we investigate the change of neurotensin solution structure induced by isotropic CHAPS-PC bicelles with and without ganglioside GM1 using solution state NMR spectroscopy. In aqueous solution the peptide is predominately unstructured. In the presence of bicelle overall structure of the peptide is stabilized. In CHAPS-PC bicelle neurotensin adopts 3(10) helical structure. In the presence of GM1 containing bicelle, the peptide adopts predominately 3(10) helical structures with small amount of α-helical structure. These results are consistent with the CD spectroscopic results. Neurotensin interacts better with GM1 containing bicelle than that of the CHAPS-PC bicelle. Docking studies between the Neurotensin Receptor3 (NTS3) and different NT conformations also indicated better binding of the NT conformation obtained in presence of GM1-containing bicelles.

PubMed: 22824320
DOI: 10.1016/j.bpc.2012.06.003

実験手法

SOLUTION NMR