2LMC

Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex

2LMC の概要

• エントリーDOI: 10.2210/pdb2lmc/pdb
• NMR情報: BMRB: 18111
• 分子名称: Bacterial RNA polymerase inhibitor, DNA-directed RNA polymerase subunit beta (2 entities in total)
• 機能のキーワード: transferase, transcription
• 由来する生物種: Enterobacteria phage T7; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18708.91

構造登録者

Liu, M. (登録日: 2011-11-29, 公開日: 2012-03-14, 最終更新日: 2024-05-01)

主引用文献

James, E.,Liu, M.,Sheppard, C.,Mekler, V.,Camara, B.,Liu, B.,Simpson, P.,Cota, E.,Severinov, K.,Matthews, S.,Wigneshweraraj, S.
Structural and Mechanistic Basis for the Inhibition of Escherichia coli RNA Polymerase by T7 Gp2.
Mol.Cell, 47:755-766, 2012

PubMed Abstract: The T7 phage-encoded small protein Gp2 is a non-DNA-binding transcription factor that interacts with the jaw domain of the Escherichia coli (Ec) RNA polymerase (RNAp) β' subunit and inhibits transcriptionally proficient promoter-complex (RPo) formation. Here, we describe the high-resolution solution structure of the Gp2-Ec β' jaw domain complex and show that Gp2 and DNA compete for binding to the β' jaw domain. We reveal that efficient inhibition of RPo formation by Gp2 requires the amino-terminal σ(70) domain region 1.1 (R1.1), and that Gp2 antagonizes the obligatory movement of R1.1 during RPo formation. We demonstrate that Gp2 inhibits RPo formation not just by steric occlusion of the RNAp-DNA interaction but also through long-range antagonistic effects on RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of R1.1 by Gp2. The inhibition of Ec RNAp by Gp2 thus defines a previously uncharacterized mechanism by which bacterial transcription is regulated by a viral factor.

PubMed: 22819324
DOI: 10.1016/j.molcel.2012.06.013

実験手法

SOLUTION NMR