2LLZ

GhoS (YjdK) monomer

2LLZ の概要

• エントリーDOI: 10.2210/pdb2llz/pdb
• NMR情報: BMRB: 18086
• 分子名称: Uncharacterized protein yjdK (1 entity in total)
• 機能のキーワード: rnase, biofilm, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11672.12

構造登録者

Lord, D.,Peti, W.,Page, R. (登録日: 2011-11-18, 公開日: 2012-09-05, 最終更新日: 2024-05-15)

主引用文献

Wang, X.,Lord, D.M.,Cheng, H.Y.,Osbourne, D.O.,Hong, S.H.,Sanchez-Torres, V.,Quiroga, C.,Zheng, K.,Herrmann, T.,Peti, W.,Benedik, M.J.,Page, R.,Wood, T.K.
A new type V toxin-antitoxin system where mRNA for toxin GhoT is cleaved by antitoxin GhoS.
Nat.Chem.Biol., 8:855-861, 2012

PubMed Abstract: Among bacterial toxin-antitoxin systems, to date no antitoxin has been identified that functions by cleaving toxin mRNA. Here we show that YjdO (renamed GhoT) is a membrane lytic peptide that causes ghost cell formation (lysed cells with damaged membranes) and increases persistence (persister cells are tolerant to antibiotics without undergoing genetic change). GhoT is part of a new toxin-antitoxin system with YjdK (renamed GhoS) because in vitro RNA degradation studies, quantitative real-time reverse-transcription PCR and whole-transcriptome studies revealed that GhoS masks GhoT toxicity by cleaving specifically yjdO (ghoT) mRNA. Alanine substitutions showed that Arg28 is important for GhoS activity, and RNA sequencing indicated that the GhoS cleavage site is rich in U and A. The NMR structure of GhoS indicates it is related to the CRISPR-associated-2 RNase, and GhoS is a monomer. Hence, GhoT-GhoS is to our knowledge the first type V toxin-antitoxin system where a protein antitoxin inhibits the toxin by cleaving specifically its mRNA.

PubMed: 22941047
DOI: 10.1038/nchembio.1062

実験手法

SOLUTION NMR