2LLI

Low resolution structure of RNA-binding subunit of the TRAMP complex

2LLI の概要

• エントリーDOI: 10.2210/pdb2lli/pdb
• NMR情報: BMRB: 18049
• 分子名称: Protein AIR2, ZINC ION (2 entities in total)
• 機能のキーワード: rna surveillance, rna degradation, rna binding, exosome, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Nucleus: Q12476
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14590.59

構造登録者

Holub, P.,Lalakova, J.,Cerna, H.,Sarazova, M.,Pasulka, J.,Hrazdilova, K.,Arce, M.S.,Stefl, R.,Vanacova, S. (登録日: 2011-11-10, 公開日: 2012-03-21, 最終更新日: 2024-05-15)

主引用文献

Holub, P.,Lalakova, J.,Cerna, H.,Pasulka, J.,Sarazova, M.,Hrazdilova, K.,Arce, M.S.,Hobor, F.,Stefl, R.,Vanacova, S.
Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation.
Nucleic Acids Res., 40:5679-5693, 2012

PubMed Abstract: Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the advances in understanding the structural features of the isolated complex subunits or their fragments, the details of complex assembly, RNA recognition and exosome activation remain poorly understood. Here we provide the first understanding of the RNA binding mode of the complex. We show that Air2p is an RNA-binding subunit of TRAMP. We identify the zinc knuckles (ZnK) 2, 3 and 4 as the RNA-binding domains, and reveal the essentiality of ZnK4 for TRAMP4 polyadenylation activity. Furthermore, we identify Air2p as the key component of TRAMP4 assembly providing bridging between Mtr4p and Trf4p. The former is bound via the N-terminus of Air2p, while the latter is bound via ZnK5, the linker between ZnK4 and 5 and the C-terminus of the protein. Finally, we uncover the RNA binding part of the Mtr4p arch, the KOW domain, as the essential component for TRAMP-mediated exosome activation.

PubMed: 22402490
DOI: 10.1093/nar/gks223

実験手法

SOLUTION NMR