2LLE

Computational design of an eight-stranded (beta/alpha)-barrel from fragments of different folds

2LLE の概要

• エントリーDOI: 10.2210/pdb2lle/pdb
• NMR情報: BMRB: 18044
• 分子名称: Chemotaxis protein CheY, Imidazole glycerol phosphate synthase subunit HisF chimera (1 entity in total)
• 機能のキーワード: (beta/alpha)8-barrel, tim-barrel, chimeric protein, computationally designed protein, lyase
• 由来する生物種: Thermotoga maritima
• 細胞内の位置: Cytoplasm: Q9X0C6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25607.60

構造登録者

Coles, M.,Truffault, V.,Eisenbeis, S.,Proffitt, W.,Meiler, J.,Hocker, B. (登録日: 2011-11-07, 公開日: 2012-03-21, 最終更新日: 2024-05-01)

主引用文献

Eisenbeis, S.,Proffitt, W.,Coles, M.,Truffault, V.,Shanmugaratnam, S.,Meiler, J.,Hocker, B.
Potential of fragment recombination for rational design of proteins.
J.Am.Chem.Soc., 134:4019-4022, 2012

PubMed Abstract: It is hypothesized that protein domains evolved from smaller intrinsically stable subunits via combinatorial assembly. Illegitimate recombination of fragments that encode protein subunits could have quickly led to diversification of protein folds and their functionality. This evolutionary concept presents an attractive strategy to protein engineering, e.g., to create new scaffolds for enzyme design. We previously combined structurally similar parts from two ancient protein folds, the (βα)(8)-barrel and the flavodoxin-like fold. The resulting "hopeful monster" differed significantly from the intended (βα)(8)-barrel fold by an extra β-strand in the core. In this study, we ask what modifications are necessary to form the intended structure and what potential this approach has for the rational design of functional proteins. Guided by computational design, we optimized the interface between the fragments with five targeted mutations yielding a stable, monomeric protein whose predicted structure was verified experimentally. We further tested binding of a phosphorylated compound and detected that some affinity was already present due to an intact phosphate-binding site provided by one fragment. The affinity could be improved quickly to the level of natural proteins by introducing two additional mutations. The study illustrates the potential of recombining protein fragments with unique properties to design new and functional proteins, offering both a possible pathway of protein evolution and a protocol to rapidly engineer proteins for new applications.

PubMed: 22329686
DOI: 10.1021/ja211657k

実験手法

SOLUTION NMR