2LKG

WSA major conformation

Summary for 2LKG

• Entry DOI: 10.2210/pdb2lkg/pdb
• Related: 2LKH
• NMR Information: BMRB: 17991
• Descriptor: Acetylcholine receptor (1 entity in total)
• Functional Keywords: transmembrane domain, nicotinic acetylcholine receptor, signaling protein
• Biological source: Torpedo marmorata
• Total number of polymer chains: 1
• Total formula weight: 15558.13

Authors

Xu, Y.,Mowrey, D.,Cui, T.,Perez-Aguilar, J.M.,Saven, J.G.,Eckenhoff, R.,Tang, P. (deposition date: 2011-10-11, release date: 2012-01-11, Last modification date: 2024-05-15)

Primary citation

Cui, T.,Mowrey, D.,Bondarenko, V.,Tillman, T.,Ma, D.,Landrum, E.,Perez-Aguilar, J.M.,He, J.,Wang, W.,Saven, J.G.,Eckenhoff, R.G.,Tang, P.,Xu, Y.
NMR structure and dynamics of a designed water-soluble transmembrane domain of nicotinic acetylcholine receptor.
Biochim.Biophys.Acta, 1818:617-626, 2011

PubMed Abstract: The nicotinic acetylcholine receptor (nAChR) is an important therapeutic target for a wide range of pathophysiological conditions, for which rational drug designs often require receptor structures at atomic resolution. Recent proof-of-concept studies demonstrated a water-solubilization approach to structure determination of membrane proteins by NMR (Slovic et al., PNAS, 101: 1828-1833, 2004; Ma et al., PNAS, 105: 16537-42, 2008). We report here the computational design and experimental characterization of WSA, a water-soluble protein with ~83% sequence identity to the transmembrane (TM) domain of the nAChR α1 subunit. Although the design was based on a low-resolution structural template, the resulting high-resolution NMR structure agrees remarkably well with the recent crystal structure of the TM domains of the bacterial Gloeobacter violaceus pentameric ligand-gated ion channel (GLIC), demonstrating the robustness and general applicability of the approach. NMR T(2) dispersion measurements showed that the TM2 domain of the designed protein was dynamic, undergoing conformational exchange on the NMR timescale. Photoaffinity labeling with isoflurane and propofol photolabels identified a common binding site in the immediate proximity of the anesthetic binding site found in the crystal structure of the anesthetic-GLIC complex. Our results illustrate the usefulness of high-resolution NMR analyses of water-solubilized channel proteins for the discovery of potential drug binding sites.

PubMed: 22155685
DOI: 10.1016/j.bbamem.2011.11.021

Experimental method

SOLUTION NMR