2LKC

Free B.st IF2-G2

2LKC の概要

• エントリーDOI: 10.2210/pdb2lkc/pdb
• 関連するPDBエントリー: 2LKD
• NMR情報: BMRB: 6946
• 分子名称: Translation initiation factor IF-2 (1 entity in total)
• 機能のキーワード: translation
• 由来する生物種: Geobacillus stearothermophilus
• 細胞内の位置: Cytoplasm: P04766
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19524.42

構造登録者

Wienk, H.,Tishchenko, E.,Belardinelli, R.,Tomaselli, S.,Dongre, R.,Spurio, R.,Folkers, G.E.,Gualerzi, C.O.,Boelens, R. (登録日: 2011-10-10, 公開日: 2012-02-15, 最終更新日: 2024-05-15)

主引用文献

Wienk, H.,Tishchenko, E.,Belardinelli, R.,Tomaselli, S.,Dongre, R.,Spurio, R.,Folkers, G.E.,Gualerzi, C.O.,Boelens, R.
Structural Dynamics of Bacterial Translation Initiation Factor IF2.
J.Biol.Chem., 287:10922-10932, 2012

PubMed Abstract: Bacterial translation initiation factor IF2 promotes ribosomal subunit association, recruitment, and binding of fMet-tRNA to the ribosomal P-site and initiation dipeptide formation. Here, we present the solution structures of GDP-bound and apo-IF2-G2 of Bacillus stearothermophilus and provide evidence that this isolated domain binds the 50 S ribosomal subunit and hydrolyzes GTP. Differences between the free and GDP-bound structures of IF2-G2 suggest that domain reorganization within the G2-G3-C1 regions underlies the different structural requirements of IF2 during the initiation process. However, these structural signals are unlikely forwarded from IF2-G2 to the C-terminal fMet-tRNA binding domain (IF2-C2) because the connected IF2-C1 and IF2-C2 modules show completely independent mobility, indicating that the bacterial interdomain connector lacks the rigidity that was found in the archaeal IF2 homolog aIF5B.

PubMed: 22308033
DOI: 10.1074/jbc.M111.333393

実験手法

SOLUTION NMR