2LJ7
3D solution structure of plant defensin Lc-def
Summary for 2LJ7
| Entry DOI | 10.2210/pdb2lj7/pdb |
| NMR Information | BMRB: 17923 |
| Descriptor | Defensin Lc-def (1 entity in total) |
| Functional Keywords | antimicrobial protein |
| Biological source | Lens culinaris subsp. culinaris (Cultivated lentil) |
| Cellular location | Secreted : B3F051 |
| Total number of polymer chains | 1 |
| Total formula weight | 5460.24 |
| Authors | Shenkarev, Z.,Mineev, K.,Gizatullina, A. (deposition date: 2011-09-07, release date: 2012-09-12, Last modification date: 2024-11-20) |
| Primary citation | Shenkarev, Z.O.,Gizatullina, A.K.,Finkina, E.I.,Alekseeva, E.A.,Balandin, S.V.,Mineev, K.S.,Arseniev, A.S.,Ovchinnikova, T.V. Heterologous expression and solution structure of defensin from lentil Lens culinaris. Biochem.Biophys.Res.Commun., 451:252-257, 2014 Cited by PubMed Abstract: A new defensin Lc-def, isolated from germinated seeds of the lentil Lens culinaris, has molecular mass 5440.4Da and consists of 47 amino acid residues. Lc-def and its (15)N-labeled analog were overexpressed in Escherichia coli. Antimicrobial activity of the recombinant protein was examined, and its spatial structure, dynamics, and interaction with lipid vesicles were studied by NMR spectroscopy. It was shown that Lc-def is active against fungi, but does not inhibit the growth of Gram-positive and Gram-negative bacteria. The peptide is monomeric in aqueous solution and contains one α-helix and triple-stranded β-sheet, which form cysteine-stabilized αβ motif (CSαβ) previously found in other plant defensins. The sterically neighboring loop1 and loop3 protrude from the defensin core and demonstrate significant mobility on the μs-ms timescale. Lc-def does not bind to the zwitterionic lipid (POPC) vesicles but interacts with the partially anionic (POPC/DOPG, 7:3) membranes under low-salt conditions. The Lc-def antifungal activity might be mediated through electrostatic interaction with anionic lipid components of fungal membranes. PubMed: 25086358DOI: 10.1016/j.bbrc.2014.07.104 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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