2LIY

Plant peptide hormone regulating stomatal density

2LIY の概要

• エントリーDOI: 10.2210/pdb2liy/pdb
• NMR情報: BMRB: 11452
• 分子名称: EPIDERMAL PATTERNING FACTOR-like protein 9 (1 entity in total)
• 機能のキーワード: plant peptide hormone, epfl family, stomatal density, positive regulator, hormone
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress)
• 細胞内の位置: Secreted (Potential): Q9SV72
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5130.78

構造登録者

Ohki, S. (登録日: 2011-09-02, 公開日: 2012-09-05, 最終更新日: 2024-10-30)

主引用文献

Ohki, S.,Takeuchi, M.,Mori, M.
The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones
Nat Commun, 2:512-512, 2011

PubMed Abstract: Stomatal development in plants is regulated by defensin-like secretory epidermal patterning factor (EPF) peptide hormones. Only one of these, stomagen, is a positive regulator, whereas EPF1, EPF2, and possibly others are negative regulators. Here we explore the structure-function relationships of EPFs, by integrating NMR and semi-in vitro stomagen experiments. We show that stomagen is composed of a loop and a scaffold containing three disulphide bonds. A mutant composed of the stomagen loop and the EPF2 scaffold positively regulates the stomatal density on Arabidopsis cotyledons. The reciprocal mutant composed of the EPF2 loop and the stomagen scaffold acts negatively. Deletion of the disulphide bond introduces unfolding and inactivity. Our results suggest that the loop confers the functional specificity of EPFs and that the scaffold is structurally required for their activity. This structural decomposition approach to elucidating the functional site could be adapted for the analysis of other cysteine-rich peptide families.

PubMed: 22027592
DOI: 10.1038/ncomms1520

実験手法

SOLUTION NMR