2LHN

RNA-binding zinc finger protein

2LHN の概要

• エントリーDOI: 10.2210/pdb2lhn/pdb
• NMR情報: BMRB: 17858
• 分子名称: Nuclear polyadenylated RNA-binding protein NAB2, ZINC ION (2 entities in total)
• 機能のキーワード: rna-binding protein, nuclear protein
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast)
• 細胞内の位置: Nucleus: P32505
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9424.02

構造登録者

Brockmann, C.,Neuhaus, D.,Stewart, M. (登録日: 2011-08-12, 公開日: 2012-06-27, 最終更新日: 2024-05-01)

主引用文献

Brockmann, C.,Soucek, S.,Kuhlmann, S.I.,Mills-Lujan, K.,Kelly, S.M.,Yang, J.C.,Iglesias, N.,Stutz, F.,Corbett, A.H.,Neuhaus, D.,Stewart, M.
Structural Basis for Polyadenosine-RNA Binding by Nab2 Zn Fingers and Its Function in mRNA Nuclear Export.
Structure, 20:1007-1018, 2012

PubMed Abstract: Polyadenylation regulation and efficient nuclear export of mature mRNPs both require the polyadenosine-RNA-binding protein, Nab2, which contains seven CCCH Zn fingers. We describe here the solution structure of fingers 5-7, which are necessary and sufficient for high-affinity polyadenosine-RNA binding, and identify key residues involved. These Zn fingers form a single structural unit. Structural coherence is lost in the RNA-binding compromised Nab2-C437S mutant, which also suppresses the rat8-2 allele of RNA helicase Dbp5. Structure-guided Nab2 variants indicate that dbp5(rat8-2) suppression is more closely linked to hyperadenylation and suppression of mutant alleles of the nuclear RNA export adaptor, Yra1, than to affinity for polyadenosine-RNA. These results indicate that, in addition to modulating polyA tail length, Nab2 has an unanticipated function associated with generating export-competent mRNPs, and that changes within fingers 5-7 lead to suboptimal assembly of mRNP export complexes that are more easily disassembled by Dbp5 upon reaching the cytoplasm.

PubMed: 22560733
DOI: 10.1016/j.str.2012.03.011

実験手法

SOLUTION NMR