2LHA

Solution structure of C2B with IP6

2LHA の概要

• エントリーDOI: 10.2210/pdb2lha/pdb
• NMR情報: BMRB: 17838
• 分子名称: Synaptotagmin-1, INOSITOL HEXAKISPHOSPHATE (2 entities in total)
• 機能のキーワード: protein-drug complex, beta-sheet protein, calcium binding protein, metal binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18012.24

構造登録者

Joung, M.,Mohan, S.K.,Yu, C. (登録日: 2011-08-08, 公開日: 2012-05-02, 最終更新日: 2024-05-15)

主引用文献

Joung, M.,Mohan, S.K.,Yu, C.
Molecular Level Interaction of Inositol Hexaphosphate with the C2B Domain of Human Synaptotagmin I
Biochemistry, 2012

PubMed Abstract: Synaptotagmin I is a synaptic vesicle membrane protein that serves as a multifunctional regulator during the exocytosis of neurotransmitter release. It contains C2A and C2B domains. The binding of Ca(2+) to the C2A domain activates the exocytosis of secretory vesicles, while the binding of inositol polyphosphates (IP4-IP6) to the C2B domain inhibits this process. To understand the IP6-induced inhibition of exocytosis of secretory vesicles, we determined the three-dimensional structure of the C2B-IP6 complex by nuclear magnetic resonance (NMR). In this study, we have determined the binding constant by isothermal titration calorimetry. The circular dichroism measurements demonstrated that IP6 can stabilize the C2B molecule. We identified the binding site using (1)H-(15)N heteronuclear single-quantum coherence spectroscopy titration data and determined the structure of the C2B-IP6 complex using multidimensional NMR studies. This information will aid in the design of better pharmacological treatments for neurological disorders.

PubMed: 22475172
DOI: 10.1021/bi300005w

実験手法

SOLUTION NMR