2LF3

Solution NMR structure of HopPmaL_281_385 from Pseudomonas syringae pv. maculicola str. ES4326, Midwest Center for Structural Genomics target APC40104.5 and Northeast Structural Genomics Consortium target PsT2A

2LF3 の概要

• エントリーDOI: 10.2210/pdb2lf3/pdb
• NMR情報: BMRB: 17737
• 分子名称: Effector protein hopAB3 (1 entity in total)
• 機能のキーワード: type iii effector, structural genomics, psi-biology, protein structure initiative, northeast structural genomics consortium, nesg, midwest center for structural genomics, mcsg, ontario centre for structural proteomics, ocsp, signaling protein
• 由来する生物種: Pseudomonas syringae pv. maculicola
• 細胞内の位置: Secreted: Q8RP04
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11767.33

構造登録者

Wu, B.,Yee, A.,Houliston, S.,Semesi, A.,Garcia, M.,Singer, A.U.,Savchenko, A.,Montelione, G.T.,Joachimiak, A.,Arrowsmith, C.H.,Northeast Structural Genomics Consortium (NESG),Midwest Center for Structural Genomics (MCSG),Ontario Centre for Structural Proteomics (OCSP) (登録日: 2011-06-28, 公開日: 2011-07-13, 最終更新日: 2024-05-15)

主引用文献

Singer, A.U.,Wu, B.,Yee, A.,Houliston, S.,Xu, X.,Cui, H.,Skarina, T.,Garcia, M.,Semesi, A.,Arrowsmith, C.H.,Savchenko, A.
Structural Analysis of HopPmaL Reveals the Presence of a Second Adaptor Domain Common to the HopAB Family of Pseudomonas syringae Type III Effectors.
Biochemistry, 51:1-3, 2012

PubMed Abstract: HopPmaL is a member of the HopAB family of type III effectors present in the phytopathogen Pseudomonas syringae. Using both X-ray crystallography and solution nuclear magnetic resonance, we demonstrate that HopPmaL contains two structurally homologous yet functionally distinct domains. The N-terminal domain corresponds to the previously described Pto-binding domain, while the previously uncharacterised C-terminal domain spans residues 308-385. While structurally similar, these domains do not share significant sequence similarity and most importantly demonstrate significant differences in key residues involved in host protein recognition, suggesting that each of them targets a different host protein.

PubMed: 22191472
DOI: 10.1021/bi2013883

実験手法

SOLUTION NMR