2LEP

Solution Structure of N-terminal Cytosolic Domain of Rhomboid Intramembrane Protease from Escherichia Coli

2LEP の概要

• エントリーDOI: 10.2210/pdb2lep/pdb
• NMR情報: BMRB: 17720
• 分子名称: Rhomboid protease glpG 1 (1 entity in total)
• 機能のキーワード: cell membrane, cytosol, membrane protein, micelles, serine protease, domain swapping, hydrolase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein (By similarity): C3SPT7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7970.89

構造登録者

Sherratt, A.R.,Ghasriani, H.,Goto, N.K. (登録日: 2011-06-20, 公開日: 2012-06-20, 最終更新日: 2024-05-01)

主引用文献

Sherratt, A.R.,Blais, D.R.,Ghasriani, H.,Pezacki, J.P.,Goto, N.K.
Activity-Based Protein Profiling of the Escherichia coli GlpG Rhomboid Protein Delineates the Catalytic Core.
Biochemistry, 51:7794-7803, 2012

PubMed Abstract: Rhomboid proteins comprise the largest class of intramembrane protease known, being conserved from bacteria to humans. The functional status of these proteases is typically assessed through direct or indirect detection of peptide cleavage products. Although these assays can report on the ability of a rhomboid to catalyze peptide bond cleavage, differences in measured hydrolysis rates can reflect changes in the structure and activity of catalytic residues, as well as the ability of the substrate to access the active site. Here we show that a highly reactive and sterically unencumbered fluorophosphonate activity-based protein profiling probe can be used to report on the catalytic integrity of active site residues in the Escherichia coli GlpG protein. We used results obtained with this probe on GlpG in proteomic samples, in combination with a conventional assay of proteolytic function on purified samples, to identify residues that are located on the cytoplasmic side of the lipid bilayer that are required for maximal proteolytic activity. Regions tested include the 90-residue aqueous-exposed N-terminus that encompasses a globular structure that we have determined by solution nuclear magnetic resonance, along with residues on the cytoplasmic side of the transmembrane domain core. While in most cases mutation or elimination of these residues did not significantly alter the catalytic status of the GlpG active site, the lipid-facing residue Arg227 was found to be important for maintaining a catalytically competent active site. In addition, we found a functionally critical region outside the transmembrane domain (TMD) core that is required for maximal protease activity. This region encompasses an additional 8-10 residues on the N-terminal side of the TMD core that precedes the first transmembrane segment and was not previously known to play a role in rhomboid function. These findings highlight the utility of the activity-based protein profiling approach for the characterization of rhomboid function.

PubMed: 22963263
DOI: 10.1021/bi301087c

実験手法

SOLUTION NMR