2LDS

Solution Structure of a Short-chain LaIT1 from the Venom of Scorpion Liocheles australasiae

2LDS の概要

• エントリーDOI: 10.2210/pdb2lds/pdb
• NMR情報: BMRB: 17681
• 分子名称: Insecticidal toxin LaIT1 (1 entity in total)
• 機能のキーワード: inhibitor cystine knot (ick) fold, scorpion toxin, toxin
• 由来する生物種: Liocheles australasiae (Wood scorpion)
• 細胞内の位置: Secreted: P0C5F2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4214.99

構造登録者

Horita, S.,Miyakawa, T.,Nagata, K.,Tanokura, M. (登録日: 2011-06-01, 公開日: 2011-09-14, 最終更新日: 2024-10-30)

主引用文献

Horita, S.,Matsushita, N.,Kawachi, T.,Ayabe, R.,Miyashita, M.,Miyakawa, T.,Nakagawa, Y.,Nagata, K.,Miyagawa, H.,Tanokura, M.
Solution structure of a short-chain insecticidal toxin LaIT1 from the venom of scorpion Liocheles australasiae.
Biochem.Biophys.Res.Commun., 411:738-744, 2011

PubMed Abstract: The solution structure of an insecticidal toxin LaIT1, a 36-residue peptide with a unique amino-acid sequence and two disulfide bonds, isolated from the venom of the scorpion Liocheles australasiae was determined by heteronuclear NMR spectroscopy. Structural similarity search showed that LaIT1 exhibits an inhibitory cystine knot (ICK)-like fold, which usually contains three or more disulfide bonds. Mutational analysis has revealed that two Arg residues of LaIT1, Arg(13) and Arg(15), play significant roles in insecticidal activity.

PubMed: 21782787
DOI: 10.1016/j.bbrc.2011.07.016

実験手法

SOLUTION NMR