2LDO

Solution structure of triheme cytochrome PpcA from Geobacter sulfurreducens reveals the structural origin of the redox-Bohr effect

2LDO の概要

• エントリーDOI: 10.2210/pdb2ldo/pdb
• NMR情報: BMRB: 16842
• 分子名称: Cytochrome c3, HEME C (2 entities in total)
• 機能のキーワード: electron transfer, electron transport
• 由来する生物種: Geobacter sulfurreducens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9603.66

構造登録者

Morgado, L.,Paixao, V.B.,Bruix, M.,Salgueiro, C.A. (登録日: 2011-05-30, 公開日: 2011-09-07, 最終更新日: 2024-11-20)

主引用文献

Morgado, L.,Paixao, V.B.,Schiffer, M.,Pokkuluri, P.R.,Bruix, M.,Salgueiro, C.A.
Revealing the structural origin of the redox-Bohr effect: the first solution structure of a cytochrome from Geobacter sulfurreducens.
Biochem.J., 441:179-187, 2012

PubMed Abstract: Gs (Geobacter sulfurreducens) can transfer electrons to the exterior of its cells, a property that makes it a preferential candidate for the development of biotechnological applications. Its genome encodes over 100 cytochromes and, despite their abundance and key functional roles, to date there is no structural information for these proteins in solution. The trihaem cytochrome PpcA might have a crucial role in the conversion of electronic energy into protonmotive force, a fundamental step for ATP synthesis in the presence of extracellular electron acceptors. In the present study, 15N-labelled PpcA was produced and NMR spectroscopy was used to determine its solution structure in the fully reduced state, its backbone dynamics and the pH-dependent conformational changes. The structure obtained is well defined, with an average pairwise rmsd (root mean square deviation) of 0.25 Å (1 Å=0.1 nm) for the backbone atoms and 0.99 Å for all heavy atoms, and constitutes the first solution structure of a Gs cytochrome. The redox-Bohr centre responsible for controlling the electron/proton transfer was identified, as well as the putative interacting regions between PpcA and its redox partners. The solution structure of PpcA will constitute the foundation for studies aimed at mapping out in detail these interacting regions.

PubMed: 21861844
DOI: 10.1042/BJ20111103

実験手法

SOLUTION NMR