2LD8

Structure of Human Telomeric DNA in Crowded Solution

2LD8 の概要

• エントリーDOI: 10.2210/pdb2ld8/pdb
• 関連するPDBエントリー: 1KF1 2gku 2JSL 2kf8 2km3
• NMR情報: BMRB: 17655
• 分子名称: Human Telomeric DNA (1 entity in total)
• 機能のキーワード: g-quadruplex, propeller-type parallel-stranded, molecular crowding, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7287.69

構造登録者

Heddi, B.,Phan, A.T. (登録日: 2011-05-17, 公開日: 2011-06-29, 最終更新日: 2024-05-15)

主引用文献

Heddi, B.,Phan, A.T.
Structure of Human Telomeric DNA in Crowded Solution
J.Am.Chem.Soc., 2011

PubMed Abstract: G-quadruplex structures formed by DNA at the human telomeres are attractive anticancer targets. Human telomeric sequences can adopt a diverse range of intramolecular G-quadruplex conformations: a parallel-stranded conformation was observed in the crystalline state, while at least four other forms were seen in K(+) solution, raising the question of which conformation is favored in crowded cellular environment. Here, we report the first NMR structure of a human telomeric G-quadruplex in crowded solution. We show that four different G-quadruplex conformations are converted to a propeller-type parallel-stranded G-quadruplex in K(+)-containing crowded solution due to water depletion. This study also reveals the formation of a new higher-order G-quadruplex structure under molecular crowding conditions. Our molecular dynamics simulations of solvent distribution provide insights at molecular level on the formation of parallel-stranded G-quadruplex in environment depleted of water. These results regarding human telomeric DNA can be extended to oncogenic promoters and other genomic G-rich sequences.

PubMed: 21548653
DOI: 10.1021/ja200786q

実験手法

SOLUTION NMR