2LC8

Solution structure of the MLV readthrough pseudoknot

2LC8 の概要

• エントリーDOI: 10.2210/pdb2lc8/pdb
• NMR情報: BMRB: 17601
• 分子名称: RNA (56-MER) (1 entity in total)
• 機能のキーワード: pseudoknot, frameshift, rna
• 由来する生物種: Moloney murine leukemia virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20348.24

構造登録者

Houck-Loomis, B.,Durney, M.A. (登録日: 2011-04-26, 公開日: 2011-11-23, 最終更新日: 2024-05-15)

主引用文献

Houck-Loomis, B.,Durney, M.A.,Salguero, C.,Shankar, N.,Nagle, J.M.,Goff, S.P.,D Souza, V.M.
An equilibrium-dependent retroviral mRNA switch regulates translational recoding
Nature, 480:561-564, 2011

PubMed Abstract: Most retroviruses require translational recoding of a viral messenger RNA stop codon to maintain a precise ratio of structural (Gag) and enzymatic (Pol) proteins during virus assembly. Pol is expressed exclusively as a Gag-Pol fusion either by ribosomal frameshifting or by read-through of the gag stop codon. Both of these mechanisms occur infrequently and only affect 5-10% of translating ribosomes, allowing the virus to maintain the critical Gag to Gag-Pol ratio. Although it is understood that the frequency of the recoding event is regulated by cis RNA motifs, no mechanistic explanation is currently available for how the critical protein ratio is maintained. Here we present the NMR structure of the murine leukaemia virus recoding signal and show that a protonation-dependent switch occurs to induce the active conformation. The equilibrium is such that at physiological pH the active, read-through permissive conformation is populated at approximately 6%: a level that correlates with in vivo protein quantities. The RNA functions by a highly sensitive, chemo-mechanical coupling tuned to ensure an optimal read-through frequency. Similar observations for a frameshifting signal indicate that this novel equilibrium-based mechanism may have a general role in translational recoding.

PubMed: 22121021
DOI: 10.1038/nature10657

実験手法

SOLUTION NMR