2LBV

Siderocalin Q83 reveals a dual ligand binding mode

2LBV の概要

• エントリーDOI: 10.2210/pdb2lbv/pdb
• NMR情報: BMRB: 17577
• 分子名称: Extracellular fatty acid-binding protein, N,N',N''-[(3S,7S,11S)-2,6,10-trioxo-1,5,9-trioxacyclododecane-3,7,11-triyl]tris(2,3-dihydroxybenzamide), GALLIUM (III) ION, ... (4 entities in total)
• 機能のキーワード: lipocalin, siderocalin, enterobactin, arachidonic acid, lipid transport
• 由来する生物種: Coturnix japonica (Japanese quail)
• 細胞内の位置: Secreted (By similarity): Q9I9P7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19148.47

構造登録者

Coudevylle, N.,Hoetzinger, M.,Geist, L.,Kontaxis, G.,Bister, K.,Konrat, R. (登録日: 2011-04-07, 公開日: 2012-02-22, 最終更新日: 2024-11-20)

主引用文献

Coudevylle, N.,Hoetzinger, M.,Geist, L.,Kontaxis, G.,Hartl, M.,Bister, K.,Konrat, R.
Lipocalin Q83 reveals a dual ligand binding mode with potential implications for the functions of siderocalins
Biochemistry, 50:9192-9199, 2011

PubMed Abstract: Siderocalins are particular lipocalins that participate in the innate immune response by interfering with bacterial siderophore-mediated iron uptake. Additionally, siderocalins are involved in several physiological and pathological processes such as inflammation, iron delivery, tissue differentiation, and cancer progression. Here we show that siderocalin Q83 displays an unexpected dual ligand binding mode as it can bind enterobactin and unsaturated fatty acids simultaneously. The solution structure of the siderocalin Q83 in complex with arachidonic acid and enterobactin reveals molecular details of this novel dual binding mode and the determinants of fatty acid binding specificity. Our results suggest that Q83 is a metabolic hub linking iron and fatty acid pathways. This unexpected coupling might contribute to the pleiotropic functions of siderocalins.

PubMed: 21951132
DOI: 10.1021/bi201115q

実験手法

SOLUTION NMR