2LAS

Molecular Determinants of Paralogue-Specific SUMO-SIM Recognition

2LAS の概要

• エントリーDOI: 10.2210/pdb2las/pdb
• 関連するPDBエントリー: 2ASQ
• NMR情報: BMRB: 17536
• 分子名称: Small ubiquitin-related modifier 1, M-IR2_peptide (2 entities in total)
• 機能のキーワード: ranbp2, post-translational modification, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus membrane: P63165
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13191.86

構造登録者

Namanja, A.,Li, Y.,Su, Y.,Wong, S.,Lu, J.,Colson, L.,Wu, C.,Li, S.,Chen, Y. (登録日: 2011-03-20, 公開日: 2011-12-14, 最終更新日: 2024-05-15)

主引用文献

Namanja, A.T.,Li, Y.J.,Su, Y.,Wong, S.,Lu, J.,Colson, L.T.,Wu, C.,Li, S.S.,Chen, Y.
Insights into High Affinity Small Ubiquitin-like Modifier (SUMO) Recognition by SUMO-interacting Motifs (SIMs) Revealed by a Combination of NMR and Peptide Array Analysis.
J.Biol.Chem., 287:3231-3240, 2012

PubMed Abstract: The small ubiquitin-like modifiers (SUMOs) regulate many essential cellular functions. Only one type of SUMO-interacting motif (SIM) has been identified that can extend the β-sheet of SUMO as either a parallel or an antiparallel strand. The molecular determinants of the bound orientation and paralogue specificity of a SIM are unclear. To address this question, we have conducted structural studies of SUMO1 in complex with a SUMO1-specific SIM that binds to SUMO1 with high affinity without post-translational modifications using nuclear magnetic resonance methods. In addition, the SIM sequence requirements have been investigated by peptide arrays in comparison with another high affinity SIM that binds in the opposing orientation. We found that antiparallel binding SIMs tolerate more diverse sequences, whereas the parallel binding SIMs prefer the more strict sequences consisting of (I/V)DLT that have a preference in high affinity SUMO2 and -3 binding. Comparison of two high affinity SUMO1-binding SIMs that bind in opposing orientations has revealed common SUMO1-specific interactions needed for high affinity binding. This study has significantly advanced our understanding of the molecular determinants underlining SUMO-SIM recognition.

PubMed: 22147707
DOI: 10.1074/jbc.M111.293118

実験手法

SOLUTION NMR