2LAC

NMR structure of unmodified_ASL_Tyr

2LAC の概要

• エントリーDOI: 10.2210/pdb2lac/pdb
• 関連するPDBエントリー: 2LA9 2LBQ 2LBR
• NMR情報: BMRB: 17520
• 分子名称: RNA (5'-R(*GP*GP*GP*GP*AP*CP*UP*GP*UP*AP*AP*AP*UP*CP*CP*CP*C)-3') (1 entity in total)
• 機能のキーワード: tyrosyl-trna, rna
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5442.30

構造登録者

Denmon, A.P.,Wang, J.,Nikonowicz, E.P. (登録日: 2011-03-10, 公開日: 2011-08-03, 最終更新日: 2024-05-15)

主引用文献

Denmon, A.P.,Wang, J.,Nikonowicz, E.P.
Conformation Effects of Base Modification on the Anticodon Stem-Loop of Bacillus subtilis tRNA(Tyr).
J.Mol.Biol., 412:285-303, 2011

PubMed Abstract: tRNA molecules contain 93 chemically unique nucleotide base modifications that expand the chemical and biophysical diversity of RNA and contribute to the overall fitness of the cell. Nucleotide modifications of tRNA confer fidelity and efficiency to translation and are important in tRNA-dependent RNA-mediated regulatory processes. The three-dimensional structure of the anticodon is crucial to tRNA-mRNA specificity, and the diverse modifications of nucleotide bases in the anticodon region modulate this specificity. We have determined the solution structures and thermodynamic properties of Bacillus subtilis tRNA(Tyr) anticodon arms containing the natural base modifications N(6)-dimethylallyl adenine (i(6)A(37)) and pseudouridine (ψ(39)). UV melting and differential scanning calorimetry indicate that the modifications stabilize the stem and may enhance base stacking in the loop. The i(6)A(37) modification disrupts the hydrogen bond network of the unmodified anticodon loop including a C(32)-A(38)(+) base pair and an A(37)-U(33) base-base interaction. Although the i(6)A(37) modification increases the dynamic nature of the loop nucleotides, metal ion coordination reestablishes conformational homogeneity. Interestingly, the i(6)A(37) modification and Mg(2+) are sufficient to promote the U-turn fold of the anticodon loop of Escherichia coli tRNA(Phe), but these elements do not result in this signature feature of the anticodon loop in tRNA(Tyr).

PubMed: 21782828
DOI: 10.1016/j.jmb.2011.07.010

実験手法

SOLUTION NMR