2LA5

RNA Duplex-Quadruplex Junction Complex with FMRP RGG peptide

2LA5 の概要

• エントリーDOI: 10.2210/pdb2la5/pdb
• NMR情報: BMRB: 17504
• 分子名称: RNA (36-MER), Fragile X mental retardation 1 protein (2 entities in total)
• 機能のキーワード: rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: Q06787
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13562.95

構造登録者

Phan, A.,Kuryavyi, V.,Darnell, J.,Serganov, A.,Majumdar, A.,Ilin, S.,Darnell, R.,Patel, D. (登録日: 2011-03-03, 公開日: 2011-06-08, 最終更新日: 2024-05-01)

主引用文献

Phan, A.T.,Kuryavyi, V.,Darnell, J.C.,Serganov, A.,Majumdar, A.,Ilin, S.,Raslin, T.,Polonskaia, A.,Chen, C.,Clain, D.,Darnell, R.B.,Patel, D.J.
Structure-function studies of FMRP RGG peptide recognition of an RNA duplex-quadruplex junction.
Nat.Struct.Mol.Biol., 18:796-804, 2011

PubMed Abstract: We have determined the solution structure of the complex between an arginine-glycine-rich RGG peptide from the human fragile X mental retardation protein (FMRP) and an in vitro-selected guanine-rich (G-rich) sc1 RNA. The bound RNA forms a newly discovered G-quadruplex separated from the flanking duplex stem by a mixed junctional tetrad. The RGG peptide is positioned along the major groove of the RNA duplex, with the G-quadruplex forcing a sharp turn of R(10)GGGGR(15) at the duplex-quadruplex junction. Arg10 and Arg15 form cross-strand specificity-determining intermolecular hydrogen bonds with the major-groove edges of guanines of adjacent Watson-Crick G•C pairs. Filter-binding assays on RNA and peptide mutations identify and validate contributions of peptide-RNA intermolecular contacts and shape complementarity to molecular recognition. These findings on FMRP RGG domain recognition by a combination of G-quadruplex and surrounding RNA sequences have implications for the recognition of other genomic G-rich RNAs.

PubMed: 21642970
DOI: 10.1038/nsmb.2064

実験手法

SOLUTION NMR