2L9L

NMR Structure of the Mouse MFG-E8 C2 Domain

2L9L の概要

• エントリーDOI: 10.2210/pdb2l9l/pdb
• NMR情報: BMRB: 17477
• 分子名称: Lactadherin (1 entity in total)
• 機能のキーワード: apoptosis, phosphatidylserine-binding protein
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Membrane; Peripheral membrane protein: P21956
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19437.76

構造登録者

Ye, H.,Yoon, H.S. (登録日: 2011-02-21, 公開日: 2012-08-29, 最終更新日: 2024-10-30)

主引用文献

Ye, H.,Li, B.,Subramanian, V.,Choi, B.H.,Liang, Y.,Harikishore, A.,Chakraborty, G.,Baek, K.,Yoon, H.S.
NMR solution structure of C2 domain of MFG-E8 and insights into its molecular recognition with phosphatidylserine
Biochim.Biophys.Acta, 1828:1083-1093, 2013

PubMed Abstract: MFG-E8 (also known as lactadherin), which is a secreted glycoprotein from a variety of cell types, possesses two EGF domains and tandem C domains with sequence homology to that of blood coagulation proteins factor V and factor VIII. MFG-E8 binds to phosphatidylserine (PS) in membranes with high affinity. We have recently shown that the C2 domain of MFG-E8 bears more specificity toward PS when compared with phosphatidylcholine (PC), another phospholipid thought to be involved in the immune function of phagocytes. In our current study, we have determined the solution structure of the C2 domain by nuclear magnetic resonance (NMR) spectroscopy, and characterized the molecular basis of binding between the C2 domain and PS by (31)P-NMR spectroscopy. Furthermore, we also verified that that positively charged and aromatic residues clustered in loops 1-3 of the C2 domain play key roles in recognizing PS in apoptotic cells.

PubMed: 23262193
DOI: 10.1016/j.bbamem.2012.12.009

実験手法

SOLUTION NMR