2L98
Structure of trans-Resveratrol in complex with the cardiac regulatory protein Troponin C
2L98 の概要
エントリーDOI | 10.2210/pdb2l98/pdb |
関連するPDBエントリー | 2kdh |
NMR情報 | BMRB: 17440 |
分子名称 | Troponin C, slow skeletal and cardiac muscles, CALCIUM ION, RESVERATROL (3 entities in total) |
機能のキーワード | structural protein, metal binding protein, contractile protein, antioxidant |
由来する生物種 | Homo sapiens (human) |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 8677.60 |
構造登録者 | Sykes, B.D.,Pineda-Sanabria, S.E.,Robertson, I.M. (登録日: 2011-02-02, 公開日: 2011-03-30, 最終更新日: 2024-05-01) |
主引用文献 | Pineda-Sanabria, S.E.,Robertson, I.M.,Sykes, B.D. Structure of trans-Resveratrol in Complex with the Cardiac Regulatory Protein Troponin C. Biochemistry, 50:1309-1320, 2011 Cited by PubMed Abstract: Cardiac troponin, a heterotrimeric protein complex that regulates heart contraction, represents an attractive target for the development of drugs for treating heart disease. Cardiovascular diseases are one of the chief causes of morbidity and mortality worldwide. In France, however, the death rate from heart disease is remarkably low relative to fat consumption. This so-called "French paradox" has been attributed to the high level of consumption of wine in France, and the antioxidant trans-resveratrol is thought to be the primary basis for wine's cardioprotective nature. It has been demonstrated that trans-resveratrol increases the myofilament Ca(2+) sensitivity of guinea pig myocytes [Liew, R., Stagg, M. A., MacLeod, K. T., and Collins, P. (2005) Eur. J. Pharmacol. 519, 1-8]; however, the specific mode of its action is unknown. In this study, the structure of trans-resveratrol free and bound to the calcium-binding protein, troponin C, was determined by nuclear magnetic resonance spectroscopy. The results indicate that trans-resveratrol undergoes a minor conformational change upon binding to the hydrophobic pocket of the C-domain of troponin C. The location occupied by trans-resveratrol coincides with the binding site of troponin I, troponin C's natural binding partner. This has been seen for other troponin C-targeting inotropes and implicates the modulation of the troponin C-troponin I interaction as a possible mechanism of action for trans-resveratrol. PubMed: 21226534DOI: 10.1021/bi101985j 主引用文献が同じPDBエントリー |
実験手法 | SOLUTION NMR |
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