2L8K

NMR Structure of the Arterivirus nonstructural protein 7 alpha (nsp7 alpha)

2L8K の概要

• エントリーDOI: 10.2210/pdb2l8k/pdb
• NMR情報: BMRB: 16977
• 分子名称: Non-structural protein 7 (1 entity in total)
• 機能のキーワード: viral protein
• 由来する生物種: Equine arteritis virus (EAV)
• 細胞内の位置: Nsp1 papain-like cysteine proteinase: Host nucleus. Nsp2 cysteine proteinase: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 3: Host membrane; Multi-pass membrane protein (Potential). Non-structural protein 5-6-7: Host membrane; Multi-pass membrane protein (Potential). 3C-like serine proteinase: Host cytoplasm (Potential). RNA-directed RNA polymerase: Host cytoplasm, host perinuclear region (Potential). Helicase: Host cytoplasm, host perinuclear region (Potential): P19811
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13489.43

構造登録者

Conte, M.R.,Gaudin, C.,Manolaridis, I.,Tucker, P.W.,Kelly, G. (登録日: 2011-01-19, 公開日: 2011-07-06, 最終更新日: 2024-05-15)

主引用文献

Manolaridis, I.,Gaudin, C.,Posthuma, C.C.,Zevenhoven-Dobbe, J.C.,Imbert, I.,Canard, B.,Kelly, G.,Tucker, P.A.,Conte, M.R.,Snijder, E.J.
Structure and Genetic Analysis of the Arterivirus Nonstructural Protein 7{alpha}.
J.Virol., 85:7449-7453, 2011

PubMed Abstract: Arterivirus replicase polyproteins are cleaved into at least 13 mature nonstructural proteins (nsps), and in particular the nsp5-to-nsp8 region is subject to a complex processing cascade. The function of the largest subunit from this region, nsp7, which is further cleaved into nsp7α and nsp7β, is unknown. Using nuclear magnetic resonance (NMR) spectroscopy, we determined the solution structure of nsp7α of equine arteritis virus, revealing an interesting unique fold for this protein but thereby providing little clue to its possible functions. Nevertheless, structure-based reverse genetics studies established the importance of nsp7/nsp7α for viral RNA synthesis, thus providing a basis for future studies.

PubMed: 21561912
DOI: 10.1128/JVI.00255-11

実験手法

SOLUTION NMR