2L8F

Structure of a 4X4 Nucleotide RNA Internal Loop from an R2 Retrotransposon

2L8F の概要

• エントリーDOI: 10.2210/pdb2l8f/pdb
• NMR情報: BMRB: 17406
• 分子名称: RNA (5'-R(*GP*UP*GP*AP*AP*GP*CP*CP*CP*GP*U)-3'), RNA (5'-R(*CP*GP*GP*AP*GP*GP*AP*CP*AP*CP*U)-3') (2 entities in total)
• 機能のキーワード: rna motif, internal loop, 3rrs, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7067.35

構造登録者

Lerman, Y.,Kennedy, S.D.,Turner, D.H. (登録日: 2011-01-11, 公開日: 2011-08-31, 最終更新日: 2024-05-15)

主引用文献

Lerman, Y.V.,Kennedy, S.D.,Shankar, N.,Parisien, M.,Major, F.,Turner, D.H.
NMR structure of a 4 x 4 nucleotide RNA internal loop from an R2 retrotransposon: Identification of a three purine-purine sheared pair motif and comparison to MC-SYM predictions.
Rna, 17:1664-1677, 2011

PubMed Abstract: The NMR solution structure is reported of a duplex, 5'GUGAAGCCCGU/3'UCACAGGAGGC, containing a 4 × 4 nucleotide internal loop from an R2 retrotransposon RNA. The loop contains three sheared purine-purine pairs and reveals a structural element found in other RNAs, which we refer to as the 3RRs motif. Optical melting measurements of the thermodynamics of the duplex indicate that the internal loop is 1.6 kcal/mol more stable at 37°C than predicted. The results identify the 3RRs motif as a common structural element that can facilitate prediction of 3D structure. Known examples include internal loops having the pairings: 5'GAA/3'AGG, 5'GAG/3'AGG, 5'GAA/3'AAG, and 5'AAG/3'AGG. The structural information is compared with predictions made with the MC-Sym program.

PubMed: 21778280
DOI: 10.1261/rna.2641911

実験手法

SOLUTION NMR