2L8C

NMR Spectroscopy and Molecular Dynamics Simulation of r(CCGCUGCGG)2 Reveal a Dynamic UU Internal Loop Found in Myotonic Dystrophy Type 1 - UU pair with zero hydrogen bond pairs

Summary for 2L8C

• Entry DOI: 10.2210/pdb2l8c/pdb
• Descriptor: RNA (5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3') (1 entity in total)
• Functional Keywords: uu mismatch, rna
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 5725.52

Authors

Parkesh, R.,Fountain, M.A.,Disney, M.D. (deposition date: 2011-01-07, release date: 2011-02-23, Last modification date: 2024-05-15)

Primary citation

Parkesh, R.,Fountain, M.,Disney, M.D.
NMR Spectroscopy and Molecular Dynamics Simulation of r(CCGCUGCGG)(2) Reveal a Dynamic UU Internal Loop Found in Myotonic Dystrophy Type 1.
Biochemistry, 50:599-601, 2011

PubMed Abstract: The NMR structure of an RNA with a copy of the 5'CUG/3'GUC motif found in the triplet repeating disorder myotonic dystrophy type 1 (DM1) is disclosed. The lowest energy conformation of the UU pair is a single-hydrogen bond structure; however, the UU protons undergo exchange indicating structural dynamics. Molecular dynamics simulations show that the single hydrogen bond structure is the most populated one but the UU pair interconverts among zero, one, and two hydrogen bond pairs. These studies have implications for the recognition of the DM1 RNA by small molecules and proteins.

PubMed: 21204525
DOI: 10.1021/bi101896j

Experimental method

SOLUTION NMR