2L8C

NMR Spectroscopy and Molecular Dynamics Simulation of r(CCGCUGCGG)2 Reveal a Dynamic UU Internal Loop Found in Myotonic Dystrophy Type 1 - UU pair with zero hydrogen bond pairs

2L8C の概要

• エントリーDOI: 10.2210/pdb2l8c/pdb
• 分子名称: RNA (5'-R(*CP*CP*GP*CP*UP*GP*CP*GP*G)-3') (1 entity in total)
• 機能のキーワード: uu mismatch, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5725.52

構造登録者

Parkesh, R.,Fountain, M.A.,Disney, M.D. (登録日: 2011-01-07, 公開日: 2011-02-23, 最終更新日: 2024-05-15)

主引用文献

Parkesh, R.,Fountain, M.,Disney, M.D.
NMR Spectroscopy and Molecular Dynamics Simulation of r(CCGCUGCGG)(2) Reveal a Dynamic UU Internal Loop Found in Myotonic Dystrophy Type 1.
Biochemistry, 50:599-601, 2011

PubMed Abstract: The NMR structure of an RNA with a copy of the 5'CUG/3'GUC motif found in the triplet repeating disorder myotonic dystrophy type 1 (DM1) is disclosed. The lowest energy conformation of the UU pair is a single-hydrogen bond structure; however, the UU protons undergo exchange indicating structural dynamics. Molecular dynamics simulations show that the single hydrogen bond structure is the most populated one but the UU pair interconverts among zero, one, and two hydrogen bond pairs. These studies have implications for the recognition of the DM1 RNA by small molecules and proteins.

PubMed: 21204525
DOI: 10.1021/bi101896j

実験手法

SOLUTION NMR