2L7D

Ribonucleotide Perturbation of DNA Structure: Solution Structure of [d(CGC)r(G)d(AATTCGCG)]2

2L7D の概要

• エントリーDOI: 10.2210/pdb2l7d/pdb
• NMR情報: BMRB: 17351
• 分子名称: 5'-D(*CP*GP*C)-R(P*G)-D(P*AP*AP*TP*TP*CP*GP*CP*G)-3' (1 entity in total)
• 機能のキーワード: ribonucleotide substituted dna, dna, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7358.78

構造登録者

DeRose, E.F.,Perera, L.,Murray, M.S.,Kunkel, T.A.,London, R.E. (登録日: 2010-12-07, 公開日: 2011-06-01, 最終更新日: 2024-05-01)

主引用文献

DeRose, E.F.,Perera, L.,Murray, M.S.,Kunkel, T.A.,London, R.E.
Solution structure of the Dickerson DNA dodecamer containing a single ribonucleotide.
Biochemistry, 51:2407-2416, 2012

PubMed Abstract: Ribonucleotides are frequently incorporated into DNA during replication. They are recognized and processed by several cellular enzymes, and their continued presence in the yeast nuclear genome results in replicative stress and genome instability. Thus, it is important to understand the effects of isolated ribonucleotide incorporation on DNA structure. With this goal in mind, we describe the nuclear magnetic resonance structure of the self-complementary Dickerson dodecamer sequence [d(CGC)rGd(AATTCGCG)](2) containing two symmetrically positioned riboguanosines. The absence of an observable H(1)-H(2) scalar coupling interaction indicates a C3'-endo conformation for the ribose. Longer-range structural perturbations resulting from the presence of the ribonucleotide are limited to the adjacent and transhelical nucleotides, while the global B-form DNA structure is maintained. Because crystallographic studies have indicated that isolated ribonucleotides promote global B → A transitions, we also performed molecular modeling analyses to evaluate the structural consequences of higher ribonucleotide substitution levels. Increasing the ribonucleotide content increased the minor groove width toward values more similar to that of A-DNA, but even 50% ribonucleotide substitution did not fully convert the B-DNA to A-DNA. Comparing our structure with the structure of an RNase H2-bound DNA supports the conclusion that, as with other DNA-protein complexes, the DNA conformation is strongly influenced by the interaction with the protein.

PubMed: 22390730
DOI: 10.1021/bi201710q

実験手法

SOLUTION NMR