2L6I

Solution structure of coronaviral stem-loop 2 (SL2)

2L6I の概要

• エントリーDOI: 10.2210/pdb2l6i/pdb
• NMR情報: BMRB: 17309
• 分子名称: RNA (5'-R(*GP*AP*UP*CP*UP*CP*UP*UP*GP*UP*AP*GP*AP*UP*CP*A)-3') (1 entity in total)
• 機能のキーワード: rna tetraloop, severe acute respiratory syndrome, mouse hepatitis virus, coronavirus, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5060.02

構造登録者

Lee, C.,Li, L.,Giedroc, D.P. (登録日: 2010-11-21, 公開日: 2011-03-23, 最終更新日: 2024-05-01)

主引用文献

Lee, C.W.,Li, L.,Giedroc, D.P.
The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold.
Febs Lett., 585:1049-1053, 2011

PubMed Abstract: The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.

PubMed: 21382373
DOI: 10.1016/j.febslet.2011.03.002

実験手法

SOLUTION NMR