2L68

Solution Structure of Human Holo L-FABP

2L68 の概要

• エントリーDOI: 10.2210/pdb2l68/pdb
• 関連するPDBエントリー: 1LFO 2F73 2JU3 2JU7 2PY1
• 分子名称: Fatty acid-binding protein, liver (1 entity in total)
• 機能のキーワード: lipid binding protein, fatty acid carrier, holo form
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P07148
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14095.17

構造登録者

Cai, J.,Luecke, C.,Chen, Z.,Qiao, Y.,Klimtchuk, E.S.,Hamilton, J.A. (登録日: 2010-11-17, 公開日: 2011-11-23, 最終更新日: 2024-05-01)

主引用文献

Cai, J.,Lucke, C.,Chen, Z.,Qiao, Y.,Klimtchuk, E.,Hamilton, J.A.
Solution structure and backbone dynamics of human liver fatty acid binding protein: fatty acid binding revisited.
Biophys.J., 102:2585-2594, 2012

PubMed Abstract: Liver fatty acid binding protein (L-FABP), a cytosolic protein most abundant in liver, is associated with intracellular transport of fatty acids, nuclear signaling, and regulation of intracellular lipolysis. Among the members of the intracellular lipid binding protein family, L-FABP is of particular interest as it can i), bind two fatty acid molecules simultaneously and ii), accommodate a variety of bulkier physiological ligands such as bilirubin and fatty acyl CoA. To better understand the promiscuous binding and transport properties of L-FABP, we investigated structure and dynamics of human L-FABP with and without bound ligands by means of heteronuclear NMR. The overall conformation of human L-FABP shows the typical β-clam motif. Binding of two oleic acid (OA) molecules does not alter the protein conformation substantially, but perturbs the chemical shift of certain backbone and side-chain protons that are involved in OA binding according to the structure of the human L-FABP/OA complex. Comparison of the human apo and holo L-FABP structures revealed no evidence for an "open-cap" conformation or a "swivel-back" mechanism of the K90 side chain upon ligand binding, as proposed for rat L-FABP. Instead, we postulate that the lipid binding process in L-FABP is associated with backbone dynamics.

PubMed: 22713574
DOI: 10.1016/j.bpj.2012.04.039

実験手法

SOLUTION NMR