2L66

The DNA-recognition fold of Sso7c4 suggests a new member of SpoVT-AbrB superfamily from archaea.

2L66 の概要

• エントリーDOI: 10.2210/pdb2l66/pdb
• 分子名称: Transcriptional regulator, AbrB family (1 entity in total)
• 機能のキーワード: dna binding protein, transcription regulator
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12304.36

構造登録者

Hsu, C.-H.,Wang, A.H.-J. (登録日: 2010-11-17, 公開日: 2011-09-07, 最終更新日: 2024-05-01)

主引用文献

Hsu, C.-H.,Wang, A.H.-J.
The DNA-recognition fold of Sso7c4 suggests a new member of SpoVT-AbrB superfamily from archaea.
Nucleic Acids Res., 39:6764-6774, 2011

PubMed Abstract: Organisms growing at elevated temperatures face the challenge of maintaining the integrity of their genetic materials. Archaea possess unique chromatin proteins for gene organization and information processing. We present the solution structure of Sso7c4 from Sulfolobus solfataricus, which has a homodimeric DNA-binding fold forming a swapped β-loop-β 'Tai-Chi' topology. The fold is reminiscent of the N-terminal DNA-binding domain of AbrB and MazE. In addition, several amide resonances in the heteronuclear single quantum coherence spectra of Sso7c4 are shifted and broadened with the addition of small amounts of duplex DNA oligomers. The locations of the corresponding amides in the Sso7c4 structure define its DNA-interacting surface. NMR spectra of DNA titrated with the protein further indicated that Sso7c4 interacts with DNA in the major groove. Taken together, a plausible model for the Sso7c4-DNA complex is presented, in which the DNA double helix is curved around the protein dimer.

PubMed: 21546550
DOI: 10.1093/nar/gkr283

実験手法

SOLUTION NMR