2L4V

Three Dimensional Structure of Pineapple Cystatin

2L4V の概要

• エントリーDOI: 10.2210/pdb2l4v/pdb
• NMR情報: BMRB: 17258
• 分子名称: Cystatin (1 entity in total)
• 機能のキーワード: cystatin, protease inhibitor, hydrolase inhibitor
• 由来する生物種: Ananas comosus (Pineapple)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15472.19

構造登録者

Chyan, C.C.L.,Deli, I.I.,Chen, B.B.J. (登録日: 2010-10-15, 公開日: 2011-10-26, 最終更新日: 2024-05-01)

主引用文献

Irene, D.,Chen, B.J.,Lo, S.H.,Liu, T.H.,Tzen, J.T.C.,Chyan, C.L.
Resonance assignments and secondary structure of a phytocystatin from Ananas comosus
Biomol.Nmr Assign., 6:99-101, 2012

PubMed Abstract: A cDNA encoding a cysteine protease inhibitor, cystatin was cloned from pineapple (Ananas comosus L.) stem. This clone was constructed into an expression vector and expressed in E. coli and purified to homogeneous. The recombinant pineapple cystatins (AcCYS) showed effectively inhibitory activity toward cysteine proteases including papain, bromelain, and cathepsin B. In order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear NMR techniques were used to characterize the structure of AcCYS. The full (1)H, (15)N, and (13)C resonance assignments of AcCYS were determined. The secondary structure of AcCYS was identified by using the assigned chemical shift of (1)Hα, (13)Cα, (13)Cβ, and (13)CO through the consensus chemical shift index (CSI). The results of CSI analysis suggest 5 β-strands (residues 45-47, 84-91, 94-104, 106-117, and 123-130) and one α-helix (residues 55-73).

PubMed: 21814766
DOI: 10.1007/s12104-011-9334-1

実験手法

SOLUTION NMR