2L41

Nab3 RRM - UCUU complex

2L41 の概要

• エントリーDOI: 10.2210/pdb2l41/pdb
• 関連するPDBエントリー: 2KVI
• 分子名称: RRM domain from Nuclear polyadenylated RNA-binding protein 3, RNA (5'-R(P*UP*CP*UP*U)-3') (2 entities in total)
• 機能のキーワード: nab3 rrm, ucuu oligonucleotide, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Saccharomyces cerevisiae (yeast)
• 細胞内の位置: Nucleus, nucleoplasm: P38996
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10007.85

構造登録者

Stefl, R.,Pergoli, R.,Hobor, F.,Kubicek, K.,Zimmermann, M.,Pasulka, J.,Hofr, C. (登録日: 2010-09-28, 公開日: 2010-11-17, 最終更新日: 2024-05-01)

主引用文献

Hobor, F.,Pergoli, R.,Kubicek, K.,Hrossova, D.,Bacikova, V.,Zimmermann, M.,Pasulka, J.,Hofr, C.,Vanacova, S.,Stefl, R.
Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (NAB) 3 protein
J.Biol.Chem., 286:3645-3657, 2011

PubMed Abstract: Non-coding RNA polymerase II transcripts are processed by the poly(A)-independent termination pathway that requires the Nrd1 complex. The Nrd1 complex includes two RNA-binding proteins, the nuclear polyadenylated RNA-binding (Nab) 3 and the nuclear pre-mRNA down-regulation (Nrd) 1 that bind their specific termination elements. Here we report the solution structure of the RNA-recognition motif (RRM) of Nab3 in complex with a UCUU oligonucleotide, representing the Nab3 termination element. The structure shows that the first three nucleotides of UCUU are accommodated on the β-sheet surface of Nab3 RRM, but reveals a sequence-specific recognition only for the central cytidine and uridine. The specific contacts we identified are important for binding affinity in vitro as well as for yeast viability. Furthermore, we show that both RNA-binding motifs of Nab3 and Nrd1 alone bind their termination elements with a weak affinity. Interestingly, when Nab3 and Nrd1 form a heterodimer, the affinity to RNA is significantly increased due to the cooperative binding. These findings are in accordance with the model of their function in the poly(A) independent termination, in which binding to the combined and/or repetitive termination elements elicits efficient termination.

PubMed: 21084293
DOI: 10.1074/jbc.M110.158774

実験手法

SOLUTION NMR