2L2R

Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)

2L2R の概要

• エントリーDOI: 10.2210/pdb2l2r/pdb
• NMR情報: BMRB: 17152
• 分子名称: Antimicrobial peptide EcAMP1 (1 entity in total)
• 機能のキーワード: disulfide-stabilized helical hairpin, antifungal peptide, antimicrobial peptide, antimicrobial protein
• 由来する生物種: Echinochloa crus-galli (cockspur grass, barnyard grass, Echinochloa crus-galli (L.) P. Beauv.)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4288.78

構造登録者

Nolde, S.B.,Barinov, N.A.,Balashova, T.A.,Arseniev, A.S.,Vassilevski, A.A.,Rogozhin, E.A.,Egorov, T.A.,Grishin, E.V. (登録日: 2010-08-26, 公開日: 2011-05-11, 最終更新日: 2024-10-09)

主引用文献

Nolde, S.B.,Vassilevski, A.A.,Rogozhin, E.A.,Barinov, N.A.,Balashova, T.A.,Samsonova, O.V.,Baranov, Y.V.,Feofanov, A.V.,Egorov, T.A.,Arseniev, A.S.,Grishin, E.V.
Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli).
J.Biol.Chem., 286:25145-25153, 2011

PubMed Abstract: This study presents purification, activity characterization, and (1)H NMR study of the novel antifungal peptide EcAMP1 from kernels of barnyard grass Echinochloa crus-galli. The peptide adopts a disulfide-stabilized α-helical hairpin structure in aqueous solution and thus represents a novel fold among naturally occurring antimicrobial peptides. Micromolar concentrations of EcAMP1 were shown to inhibit growth of several fungal phytopathogens. Confocal microscopy revealed intensive EcAMP1 binding to the surface of fungal conidia followed by internalization and accumulation in the cytoplasm without disturbance of membrane integrity. Close spatial structure similarity between EcAMP1, the trypsin inhibitor VhTI from seeds of Veronica hederifolia, and some scorpion and cone snail toxins suggests natural elaboration of different functions on a common fold.

PubMed: 21561864
DOI: 10.1074/jbc.M110.200378

実験手法

SOLUTION NMR