2L2K

Solution NMR structure of the R/G STEM LOOP RNA-ADAR2 DSRBM2 Complex

2L2K の概要

• エントリーDOI: 10.2210/pdb2l2k/pdb
• 関連するPDBエントリー: 2L2J
• 分子名称: Adenosine deaminase, RNA (42-MER) (2 entities in total)
• 機能のキーワード: ac mismatch, rna editing, dsrbm, stemloop, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Mus musculus (mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21173.85

構造登録者

Allain, F.H.-T.,Oberstrass, F.C.,Stefl, R. (登録日: 2010-08-20, 公開日: 2010-10-27, 最終更新日: 2024-05-01)

主引用文献

Stefl, R.,Oberstrass, F.C.,Hood, J.L.,Jourdan, M.,Zimmermann, M.,Skrisovska, L.,Maris, C.,Peng, L.,Hofr, C.,Emeson, R.B.,Allain, F.H.
The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove.
Cell(Cambridge,Mass.), 143:225-237, 2010

PubMed Abstract: Sequence-dependent recognition of dsDNA-binding proteins is well understood, yet sequence-specific recognition of dsRNA by proteins remains largely unknown, despite their importance in RNA maturation pathways. Adenosine deaminases that act on RNA (ADARs) recode genomic information by the site-selective deamination of adenosine. Here, we report the solution structure of the ADAR2 double-stranded RNA-binding motifs (dsRBMs) bound to a stem-loop pre-mRNA encoding the R/G editing site of GluR-2. The structure provides a molecular basis for how dsRBMs recognize the shape, and also more surprisingly, the sequence of the dsRNA. The unexpected direct readout of the RNA primary sequence by dsRBMs is achieved via the minor groove of the dsRNA and this recognition is critical for both editing and binding affinity at the R/G site of GluR-2. More generally, our findings suggest a solution to the sequence-specific paradox faced by many dsRBM-containing proteins that are involved in post-transcriptional regulation of gene expression.

PubMed: 20946981
DOI: 10.1016/j.cell.2010.09.026

実験手法

SOLUTION NMR