2L1V

Solution structure of a preQ1 riboswitch (Class I) aptamer bound to preQ1

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2L1V の概要

• エントリーDOI: 10.2210/pdb2l1v/pdb
• NMR情報: BMRB: 17106
• 分子名称: 36-MER, 7-DEAZA-7-AMINOMETHYL-GUANINE (2 entities in total)
• 機能のキーワード: preq1, riboswitch, rna, aptamer, pseudoknot
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11707.08

構造登録者

Kang, M.,Zhang, Q.,Feigon, J. (登録日: 2010-08-06, 公開日: 2010-09-01, 最終更新日: 2024-05-15)

主引用文献

Kang, M.,Peterson, R.D.,Feigon, J.
Structural Insights into Riboswitch Control of the Biosynthesis of Queuosine, a Modified Nucleotide Found in the Anticodon of tRNA
Mol.Cell, 33:784-790, 2009

PubMed Abstract: The modified nucleotide queuosine (Q) is almost universally found in the anticodon wobble position of specific tRNAs. In many bacteria, biosynthesis of Q is modulated by a class of regulatory mRNA elements called riboswitches. The preQ(1) riboswitch, found in the 5'UTR of bacterial genes involved in synthesis of the Q precursors preQ(0) and preQ(1), contains the smallest known aptamer domain. We report the solution structure of the preQ(1) riboswitch aptamer domain from Bacillus subtilis bound to preQ(1), which is a unique compact pseudoknot with three loops and two stems that encapsulates preQ(1) at the junction between the two stems. The pseudoknot only forms in the presence of preQ(1), and the 3' A-rich tail of the aptamer domain is an integral part of the pseudoknot. In the absence of preQ(1), the A-rich tail forms part of the antiterminator. These structural studies provide insight into riboswitch transcriptional control of preQ(1) biosynthesis.

PubMed: 19285444
DOI: 10.1016/j.molcel.2009.02.019

実験手法

SOLUTION NMR