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2L1F

Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography

2L1F の概要
エントリーDOI10.2210/pdb2l1f/pdb
NMR情報BMRB: 17083
分子名称RNA (65-MER), RNA (66-MER) (2 entities in total)
機能のキーワードrna, retrovirus, packaging, cryo-et
由来する生物種Moloney Murine Leukemia Virus
詳細
タンパク質・核酸の鎖数2
化学式量合計42488.47
構造登録者
主引用文献Miyazaki, Y.,Irobalieva, R.N.,Tolbert, B.S.,Smalls-Mantey, A.,Iyalla, K.,Loeliger, K.,D'Souza, V.,Khant, H.,Schmid, M.F.,Garcia, E.L.,Telesnitsky, A.,Chiu, W.,Summers, M.F.
Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography.
J.Mol.Biol., 404:751-772, 2010
Cited by
PubMed Abstract: The 5'-untranslated regions of all gammaretroviruses contain a conserved "double-hairpin motif" (Ψ(CD)) that is required for genome packaging. Both hairpins (SL-C and SL-D) contain GACG tetraloops that, in isolated RNAs, are capable of forming "kissing" interactions stabilized by two intermolecular G-C base pairs. We have determined the three-dimensional structure of the double hairpin from the Moloney murine leukemia virus ([Ψ(CD)](2), 132 nt, 42.8 kDa) using a (2)H-edited NMR-spectroscopy-based approach. This approach enabled the detection of (1)H-(1)H dipolar interactions that were not observed in previous studies of isolated SL-C and SL-D hairpin RNAs using traditional (1)H-(1)H correlated and (1)H-(13)C-edited NMR methods. The hairpins participate in intermolecular cross-kissing interactions (SL-C to SL-D' and SLC' to SL-D) and stack in an end-to-end manner (SL-C to SL-D and SL-C' to SL-D') that gives rise to an elongated overall shape (ca 95 Å×45 Å×25 Å). The global structure was confirmed by cryo-electron tomography (cryo-ET), making [Ψ(CD)](2) simultaneously the smallest RNA to be structurally characterized to date by cryo-ET and among the largest to be determined by NMR. Our findings suggest that, in addition to promoting dimerization, [Ψ(CD)](2) functions as a scaffold that helps initiate virus assembly by exposing a cluster of conserved UCUG elements for binding to the cognate nucleocapsid domains of assembling viral Gag proteins.
PubMed: 20933521
DOI: 10.1016/j.jmb.2010.09.009
主引用文献が同じPDBエントリー
実験手法
SOLUTION NMR
構造検証レポート
Validation report summary of 2l1f
検証レポート(詳細版)ダウンロードをダウンロード

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件を2026-02-04に公開中

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