2L15

Solution Structure of Cold Shock Protein CspA Using Combined NMR and CS-Rosetta method

2L15 の概要

• エントリーDOI: 10.2210/pdb2l15/pdb
• 分子名称: Cold shock protein CspA (1 entity in total)
• 機能のキーワード: cspa, cs-rosetta, transcription
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm (By similarity): Q0TBP6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7411.25

構造登録者

Tang, Y.,Schneider, W.M.,Shen, Y.,Raman, S.,Inouye, M.,Baker, D.,Roth, M.J.,Montelione, G.T. (登録日: 2010-07-22, 公開日: 2010-09-15, 最終更新日: 2024-05-01)

主引用文献

Tang, Y.,Schneider, W.M.,Shen, Y.,Raman, S.,Inouye, M.,Baker, D.,Roth, M.J.,Montelione, G.T.
Fully automated high-quality NMR structure determination of small (2)H-enriched proteins.
J Struct Funct Genomics, 11:223-232, 2010

PubMed Abstract: Determination of high-quality small protein structures by nuclear magnetic resonance (NMR) methods generally requires acquisition and analysis of an extensive set of structural constraints. The process generally demands extensive backbone and sidechain resonance assignments, and weeks or even months of data collection and interpretation. Here we demonstrate rapid and high-quality protein NMR structure generation using CS-Rosetta with a perdeuterated protein sample made at a significantly reduced cost using new bacterial culture condensation methods. Our strategy provides the basis for a high-throughput approach for routine, rapid, high-quality structure determination of small proteins. As an example, we demonstrate the determination of a high-quality 3D structure of a small 8 kDa protein, E. coli cold shock protein A (CspA), using <4 days of data collection and fully automated data analysis methods together with CS-Rosetta. The resulting CspA structure is highly converged and in excellent agreement with the published crystal structure, with a backbone RMSD value of 0.5 Å, an all atom RMSD value of 1.2 Å to the crystal structure for well-defined regions, and RMSD value of 1.1 Å to crystal structure for core, non-solvent exposed sidechain atoms. Cross validation of the structure with (15)N- and (13)C-edited NOESY data obtained with a perdeuterated (15)N, (13)C-enriched (13)CH(3) methyl protonated CspA sample confirms that essentially all of these independently-interpreted NOE-based constraints are already satisfied in each of the 10 CS-Rosetta structures. By these criteria, the CS-Rosetta structure generated by fully automated analysis of data for a perdeuterated sample provides an accurate structure of CspA. This represents a general approach for rapid, automated structure determination of small proteins by NMR.

PubMed: 20734145
DOI: 10.1007/s10969-010-9095-6

実験手法

SOLUTION NMR